The anti-IgE autoantibody(anti-IgE) in the IgG class was measured in children with atopic or non-atopic asthma using solid phase enzme-linked immunosorbent assay(ELISA) in avidine-biotin systme.
The IgG anti-IgE was detected in 74% of children with atopic asthma, and in 83.3% of those with non-atopic asthma. In atopic asthmatics, children with the relatively high IgE antibody level (more than 100 IU /ml) showed 83.3% of positive rate of
IgG
anti-IgE, and those with relatively low IgE level (less than 100IU /ml) showed 55.6% of positivity. In non-atopic asthmatics the positivity. In non-atopic asthmatics the positive rates of IgG anti-IgE were 100% and 77.7% in the children with high
and
low IgE levels, respectively.
The IgG1 and IgG4 antibodies against IgE were also measured in all children, and there were no significant differences of positive rate and relative concentration of IgG1, IgG4 anti-IgE between atopic and non-atopic asthmatics.. The concentration
of
IgG, IgG1 and IgG anti-IgE were well correlated with the level of totlal serum IgE antibody(r=0.33 r=0.39 and r=0.36 and r=0.36 : p < 0.05) and there was also good correlation between igG1 or IgG 4 anti-IgE and total IgG anti-IgE(r=0.84, r=0.83 ;
p
<0.0001).
In summary there were no differences in positive rate and relative concentrations of IgG, IgG1 and IgG4 anti-IgE autoantibodies between atopic and non-atopic asthmatic children. And there was good correlations among IgG, IgG1 or IgG4 anti-IgE and
total
serum IgE antibody.
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